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KMID : 0545120140240070954
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 7 p.954 ~ p.958
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Kinetic Characterization and Molecular Modeling of NAD(P)+-Dependent Succinic Semialdehyde Dehydrogenase from Bacillus subtilis as an Ortholog YneI
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Park Seong-Ah
Park Ye-Song
Lee Ki-Seog
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Abstract
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Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde (SSA) into succinic acid in the final step of γ-aminobutyric acid degradation. Here, we characterized Bacillus subtilis SSADH (BsSSADH) regarding its cofactor discrimination and substrate inhibition. BsSSADH showed similar values of the catalytic efficiency (kcat/Km) in both NAD+ and NADP+ as cofactors, and exhibited complete uncompetitive substrate inhibition at higher SSA concentrations. Further analyses of the sequence alignment and homology modeling indicated that the residues of catalytic and cofactor-binding sites in other SSADHs were highly conserved in BsSSADH.
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KEYWORD
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Succinic semialdehyde dehydrogenase, Bacillus subtilis YneI, γ-Aminobutyric acid degradation, Cofactor preference, Substrate inhibition
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